human npnt expression (TaKaRa)
Structured Review

Human Npnt Expression, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 593 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human npnt expression/product/TaKaRa
Average 95 stars, based on 593 article reviews
Images
1) Product Images from "Antibodies against nephronectin ameliorate anti‐type II collagen‐induced arthritis in mice"
Article Title: Antibodies against nephronectin ameliorate anti‐type II collagen‐induced arthritis in mice
Journal: FEBS Open Bio
doi: 10.1002/2211-5463.12758
Figure Legend Snippet: Real‐time PCR primer sequences.
Techniques Used: Real-time Polymerase Chain Reaction, Sequencing
Figure Legend Snippet: Primer sequences for cloning of Npnt.
Techniques Used: Cloning, Sequencing
Figure Legend Snippet: Npnt neutralization by anti‐Npnt‐FD antibody ameliorates CAIA. (A) Protocol for CAIA with anti‐Npnt‐FD antibody treatment. The antibody was administered to BALB/c mice on days −4 and 0 relative to LPS injection during CAIA induction. (B) Npnt expression in arthritic joints on day 6, synovial fibroblasts, or synovial macrophages were evaluated by real‐time PCR. * P < 0.05, ** P < 0.01, wild‐type versus CAIA (Student's t ‐test). (C) Arthritis scores of arthritic mice treated with control antibody (rabbit IgG) or an anti‐Npnt‐FD antibody at the indicated time points ( n = 5 per group). * P < 0.05, ** P < 0.01, anti‐Npnt‐FD antibody versus control Ig (Student's t ‐test). Data are presented as means ± SEM. (D) Representative images of the forepaw and hindpaw on day 7 are shown. (E–G) Representative histology of normal joints and arthritic joints on day 14 from mice treated with control antibody or anti‐Npnt‐FD antibody. Sections were stained with HE (E, G) or Safranin‐O (F). Magnified views of the boxed areas a and b are shown in F and G, respectively. Scale bar = 2mm (E), 200μm (F and G). Data are representative of three or more independent experiments with similar results.
Techniques Used: Neutralization, Injection, Expressing, Real-time Polymerase Chain Reaction, Control, Staining